Sanger sequencing

Sanger sequencing



Sanger sequencing can be used to determine the sequence of PCR products or vectors, obtaining yields of up to 1000 bp. Indicated for:

  • PCR products, vectors (DNA cloned in plasmid, phage, BAC ...).
  • Sequence simple genes.
  • Cost-effective sequencing of individual samples.
  • Verification of directed mutagenesis or presence of cloned inserts.

We use BigDye Terminator chemistry v.3.1 (Applied Byosistems) and AbiPrism technology (Thermo Fisher Scientific)



Rates Taxes excluded

50

Sequencing (purified DNA, primers supplied by the client *)

14 € **

ANTES 28 €

855 € **(9 €/sample)

ANTES 1.710 € (18 €/sample)

Only migrate (DNA, first, BDT 3.1 supplied by the client)

7 €

380 € (4 €/sample)


* General recommendation: it is recommended to mix 10 μL of purified DNA (PCR products> 20 ng / μL, plasmids 100-500 ng / μL) and 3 μL of primer (10 pmol / μL). Consult us in case of doubt at the time of preparing the samples.

In case of sequencing failures due to the characteristics of the sample (low quality of DNA or quantity, poorly purified or inadequate primer) the services will be billed as well.


** 5th anniversary promotion

Promotion valid for the first order and with a minimum amount of 18,500 € of tax base. Ask us for any clarification

Sending samples

Samples must be correctly labeled and identified. It is recommended to use well sealed 0.2 mL tubes. The PCR or DNA products to be sequenced must be purified and quantified (see if you need these services), and the samples must be diluted in nuclease-free water, avoiding elution buffers from commercial kits.

Request for a quote

Request

Depending on the needs of each project, BIOGENETICS will study your case to offer the most suitable budget. BIOGENETICS offers special promotions in case of contracting large volumes or multiple analyzes. Ask us to know more about the promotions.