Sanger sequencing can be used to determine the sequence of PCR products or vectors, obtaining yields of up to 1000 bp. Indicated for:
We use BigDye Terminator chemistry v.3.1 (Applied Byosistems) and AbiPrism technology (Thermo Fisher Scientific)
Rates Taxes excluded
Sequencing (purified DNA, primers supplied by the client *)
Only migrate (DNA, first, BDT 3.1 supplied by the client)
* General recommendation: it is recommended to mix 10 μL of purified DNA (PCR products> 20 ng / μL, plasmids 100-500 ng / μL) and 3 μL of primer (10 pmol / μL). Consult us in case of doubt at the time of preparing the samples.
In case of sequencing failures due to the characteristics of the sample (low quality of DNA or quantity, poorly purified or inadequate primer) the services will be billed as well.